Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D-WM-E2-ER
cell line
T47D breast carcinoma cell line
agent
Estrogen
chip antibody
ER (Ab10 Thermo Fisher Scientific, SC-543 Santa cruz)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted with a sarkosyl based buffer and sonicated to a size range of 200-300 bp DNA sequencing libraries were prepared using the ThruPLEX-FD Prep Kit (Takara Bio USA)

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
41696011
Reads aligned (%)
97.4
Duplicates removed (%)
10.3
Number of peaks
4229 (qval < 1E-05)

hg19

Number of total reads
41696011
Reads aligned (%)
96.4
Duplicates removed (%)
12.1
Number of peaks
3840 (qval < 1E-05)

Base call quality data from DBCLS SRA