GSM4589492: 40 K562 ASTAR-ATAC-5-11; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
K562
cell line
K562
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Single cell suspensions were harvested for ASTAR Scripts run using C1 Fluidigm Open App chips (10-17um). The open chromatin was transposed by Tn5 transposase. Cell specific barcode was integrated to tagmented DNA of each cell by PCR amplification with indexed primer. The amplified PCR produced was purified by Ampure XP beads and the purified ATACseq library was sent for sequencing.