Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
iPSC
clone
WT_B
differentiation
Undifferentiated
treatment
RNaseAneg
molecule subtype
DNA pulldown by RPB1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA or RNA samples in fRIP-seq were extracted with Trizol following manufactuer's protocol. DNA samples in ChIP-seq was extracted using phenol-chloroform. KAPA RNA Hyperprep kit with riboerase (RNA-seq and fRIP-seq), KAPA HyperPlus Kit (ChIP-seq)

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
33780279
Reads aligned (%)
98.6
Duplicates removed (%)
18.8
Number of peaks
16726 (qval < 1E-05)

hg19

Number of total reads
33780279
Reads aligned (%)
98.1
Duplicates removed (%)
19.4
Number of peaks
16689 (qval < 1E-05)

Base call quality data from DBCLS SRA