GSM4577177: PT1 ESR1 ChIP Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Uterus
Cell type
Endometrial stromal cells
NA
NA
Attributes by original data submitter
Sample
source_name
Isolated stromal cells post-hysterectomy
cell type
Primary endometrial stromal cells
individual
Patient 1
antibody target
input
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
7.5e8 cells (patient number = 1) were used for ChIP-Seq using the SimpleChIP Kit (Cell Signaling Technology, #9005). ESR1 antibody was purchased from Santa Cruz (#sc-543). Next-generation sequencing libraries were prepared using the KAPA Hyper Prep Kit (KAPA Biosystems, KK8502) and KAPA Single-Indexed Adapter Kit (KAPA Biosystems, KK8710).