Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TAZ

Cell type

Cell type Class
Lung
Cell type
NCI-H1299
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma Non-Small Cell

Attributes by original data submitter

Sample

source_name
H1299 cells
cell type
non-small cell lung cancer
cell line
H1299
antibodies
Anti-TAZ

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei. The chromatin solution was immunoprecipitated with either YAP antibody or TAZ antibody. The immunoprecipitations were performed using Pierce ChIP-grade Protein G magnetic beads (Thermo Fisher Scientific). Reverse cross-linking and DNA purification was performed by ChIP DNA Clean & Concentrator kit (Zymo Research, USA). Libraries were prepared as described in Blecher-Gonen et al., Nat Protoc, 2013. 8(3): p. 539-54.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
27376067
Reads aligned (%)
69.9
Duplicates removed (%)
23.7
Number of peaks
4242 (qval < 1E-05)

hg38

Number of total reads
27376067
Reads aligned (%)
74.0
Duplicates removed (%)
20.6
Number of peaks
3893 (qval < 1E-05)

Base call quality data from DBCLS SRA