Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Thymus

NA

NA

Sample

source_name

Human postnatal thymus

tissue

postnatal thymus

chip antibody

H3K27ac (Diagenode, C15410174)

cell population

CD34+ CD1+

donor

D2

sort phenotype

CD34+ CD4- CD1+

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

50-100 000 sorted, fixed cells were lysed in lysis buffer complemented with 20mM NaBU HDAC inhibitor and sheared on the Bioruptor Pico (Diagenode). Sheared chromatin was magnetically immunoprecipitated and tagmentated using the Auto ChIPmentation Kit for Histones (Diagenode). Libraries were constructed with the Auto ChIPmentation Kit for histones (Diagenode). Libraries were sequenced with the Nextseq500 (SR75, High Output).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

24984992

Reads aligned (%)

72.6

Duplicates removed (%)

16.2

Number of peaks

792 (qval < 1E-05)

hg19

Number of total reads

24984992

Reads aligned (%)

71.9

Duplicates removed (%)

17.5

Number of peaks

787 (qval < 1E-05)