GSM4559739: NGP ctrl ARID1A; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ARID1A
Cell type
Cell type Class
Neural
Cell type
NGP
NA
NA
Attributes by original data submitter
Sample
source_name
Neuroblastoma
cell line
NGP
genotype
control
chip antiboy
ARID1A (EMD Millipore, #04-080)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and target bound fragments were isolated with antibody. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.