Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AHR

Cell type

Cell type Class
Cardiovascular
Cell type
HCASMC
NA
NA

Attributes by original data submitter

Sample

source_name
HCASMC_AHR ChIP-Seq
cell line
Human Coronary Artery Smooth Muscle Cells (HCASMC)
tissue
coronary artery
genotype/treatment
dioxin (TCDD) 10nM
chip antibody
AHR antibody (Santa Cruz, sc-5579)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed using AHR antibody (Santa Cruz, sc-5579) in HCASMC between passages 5-8 in the presence of 10nM of TCDD, and library was constructed using the KAPA Hyper Prep Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
40138701
Reads aligned (%)
145.6
Duplicates removed (%)
11.2
Number of peaks
18081 (qval < 1E-05)

hg38

Number of total reads
40138701
Reads aligned (%)
148.8
Duplicates removed (%)
10.7
Number of peaks
18701 (qval < 1E-05)

Base call quality data from DBCLS SRA