Antigen

Antigen Class

TFs and others

Antigen

AHR

Cell type

Cell type Class

Cardiovascular

Cell type

HCASMC

NA

NA

Sample

source_name

HCASMC_AHR ChIP-Seq

cell line

Human Coronary Artery Smooth Muscle Cells (HCASMC)

tissue

coronary artery

genotype/treatment

dioxin (TCDD) 10nM

chip antibody

AHR antibody (Santa Cruz, sc-5579)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed using AHR antibody (Santa Cruz, sc-5579) in HCASMC between passages 5-8 in the presence of 10nM of TCDD, and library was constructed using the KAPA Hyper Prep Kit.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

40138701

Reads aligned (%)

83.3

Duplicates removed (%)

10.7

Number of peaks

18701 (qval < 1E-05)

hg19

Number of total reads

40138701

Reads aligned (%)

82.0

Duplicates removed (%)

11.2

Number of peaks

18081 (qval < 1E-05)