GSM4462998: Cyt49 PP2.ChIP-Seq.GATA6.replicate.2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
GATA6
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC derived pancreatic cells
NA
NA
Attributes by original data submitter
Sample
source_name
Cyt49 Control
cell line
Cyt49
cell type
differentiated hESCs
developmental stage
Late pancreatic progenitor
chip antibody
Rabbit anti-GATA6
antibody manufacturer
Santa Cruz Biotechnology
antibody catalog number
SC-9055
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed using the ChIP-IT High-Sensitivity kit (Active Motif) according to the manufacturer's instructions. Briefly, for each cell stage and condition analyzed, 5-10 x 10e6 cells were harvested and fixed for 15 min in an 11.1% formaldehyde solution. Cells were lysed and homogenised using a Dounce homogeniser and the lysate was sonicated in a Bioruptor® Plus (Diagenode), on high for 3 x 5 min (30 sec on, 30 sec off). Between 10 and 30 µg of the resulting sheared chromatin was used for each immunoprecipitation. Libraries were prepared using the KAPA DNA Library Preparation Kits for Illumina® Kit Libraries were prepared using the TruSeq® Stranded mRNA Library Prep Kit (RNA-seq) and the KAPA DNA Library Preparation Kits for Illumina® KAPA Kit (ChIP-seq)