Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Neural
Cell type
U-87 MG
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma

Attributes by original data submitter

Sample

source_name
Glioblastoma cell line
cell type
U87
treatment
Panobinostat
chip antibody
H3k27me3 (Cell Signalling 9733)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 from Cell Signalling Next generation sequencing (NGS) from Genewiz, New Jersey

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
62593364
Reads aligned (%)
99.0
Duplicates removed (%)
23.3
Number of peaks
578 (qval < 1E-05)

hg19

Number of total reads
62593364
Reads aligned (%)
98.4
Duplicates removed (%)
23.9
Number of peaks
778 (qval < 1E-05)

Base call quality data from DBCLS SRA