GSM4546483: SK-N-BE(2)-C genomic input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Neural
Cell type
SK-N-BE(2)
Primary Tissue
Brain
Site of Extraction
Bone Marrow
Tissue Diagnosis
Neuroblastoma
Attributes by original data submitter
Sample
source_name
Neuroblastoma tumour derived
tissue
Neuroblastoma
cell_line
SK-N-BE(2)-C
genomic_features
MYCN_amp, 17q gain
anitbody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
SK-N-BE(2)-C cells (1x10^7) were harvested and lysed, lysates were then clarified from sonicated nuclei and histone-DNA complexes were isolated using chromatin immunoprecipitation with a mouse anti-ALYREF antibody (11G5, ImmunoQuest) or control mouse IgG (Santa Cruz). Sequencing libraries were created using an Illumina TruePrep DNA Library Prep Kit (TD501) following manufacturers instructions.