Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Breast
Cell type
Mammary epithelial cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mammary epithelial cells
cell line
Comma-Dbeta
passage number
p28
chip antibody
H3K27Ac (Active Motif 39034)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
chromatin was immunoprecipitated from cross-linked nuclear extracts using antibodies and DNA was purified. Illumina TruSeq ChIP library prep kit (Illumina)

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
7769630
Reads aligned (%)
85.7
Duplicates removed (%)
64.3
Number of peaks
46 (qval < 1E-05)

mm9

Number of total reads
7769630
Reads aligned (%)
85.1
Duplicates removed (%)
64.6
Number of peaks
39 (qval < 1E-05)

Base call quality data from DBCLS SRA