Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
msl-1

Cell type

Cell type Class
Larvae
Cell type
Salivary glands
NA
NA

Attributes by original data submitter

Sample

source_name
salivary glands
developmental stage
third instar
Sex
male
chip antibody
polyclonal MSL1 antibody raised against the N-terminus (AA1-584)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was obtained from male 3rd instar larvae salivary glands as previously described (PMID: 16547175). In brief, collected salivary glands (10 per ChIP) were fixed, sonicated for 8 min (pulsed eight times for 30 sec, paused for 30 sec, high) in a Bioruptor (Cosmo Bio), and chromatin immunoprecipitated with the respective antibody.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

dm3

Number of total reads
19577660
Reads aligned (%)
33.0
Duplicates removed (%)
39.6
Number of peaks
5550 (qval < 1E-05)

dm6

Number of total reads
19577660
Reads aligned (%)
32.1
Duplicates removed (%)
41.2
Number of peaks
5177 (qval < 1E-05)

Base call quality data from DBCLS SRA