Antigen

Antigen Class

Histone

Antigen

H4K16ac

Cell type

Cell type Class

Gonad

Cell type

KGN

Primary Tissue

Ovary

Tissue Diagnosis

Tumor Granulosa Cell

Sample

source_name

human ovarian granulosa KGN cells

cell line

KGN

cell type

Ovarian granulosa cells

passages

5-8

treatment

Control

chip antibody

H4K16ac (CST, Cat# E2B8W)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

We used ChIP-seq kit (CST, Cat# 9003) to extract chromatins following the manufacturer instructions. Libraries were prepared according to Illumina's protocols. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

48277642

Reads aligned (%)

97.0

Duplicates removed (%)

14.2

Number of peaks

475 (qval < 1E-05)

hg19

Number of total reads

48277642

Reads aligned (%)

96.7

Duplicates removed (%)

14.8

Number of peaks

243 (qval < 1E-05)