Antigen

Antigen Class

TFs and others

Antigen

SUMO2

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

Isogenic HEK293 cells, 3x-FLAG-PIAS1, tet, SUMO-2/3 ChIP

cell line

HEK293

cell type

human embryonal kidney cell line

stably expressing

3x-FLAG-PIAS1

treatment

tetracycline

concentration

100 ng/ml

time

24 h

chip antibody

SUMO-2/3 (MBL International, M114-3, lot # 24)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated with 1 µg of antibody. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

192708750

Reads aligned (%)

97.8

Duplicates removed (%)

35.1

Number of peaks

111592 (qval < 1E-05)

hg19

Number of total reads

192708750

Reads aligned (%)

97.2

Duplicates removed (%)

36.8

Number of peaks

110227 (qval < 1E-05)