GSM4498323: ChIP-seq H3K27ac non treat; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Neural
Cell type
Hippocampal neurons
NA
NA
Attributes by original data submitter
Sample
source_name
Treatment: non
strain
ICR
tissue
E17 hippocampus
cell type
Cultured hippocampal neurons (10DIV)
chip antibody
mouse monoclonal anti-H3K27ac; MBL
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Bisulfite-Seq; Genomic DNA was extracted using Phenol:Chloroform:Isoamyl Alcohol 25:24:1 (Nacalai Tesque). RNA-seq; Total RNAs were isolated with Sepasol-RNA I Super G (Nacalai Tesque), Total RNAs were purified using Poly (A) mRNA Magnetic Isolation Module (New England Biolabs). ChIP-seq; Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Bisulfite-Seq; Genomic DNA was subjected to bisulfite treatment with the EZ DNA Methylation-Gold Kit (ZYMO Research) according to the manufacturer's instructions. In this study, we adopted a strategy termed post-bisulfite adaptor tagging (PBAT), which provides an unbiased methylome analysis (Miura et al. 2012). The multiplex PBAT method is described in detail on the CREST/IHEC Japan webpage (http://crest-ihec.jp/english/epigenome/index.html) (Miura et al. 2012). RNA-seq; Purified mRNAs by polyA beads were used for library construction with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). ChIP-seq; Sequencing libraries were made using the NEBNext ultra II DNA Library Prep kit for Illumina (New England Biolabs).