GSM4491170: RS411 NT-B K9ac; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Blood
Cell type
RS 411
NA
NA
Attributes by original data submitter
Sample
source_name
B-ALL cell line
tissue
bone marrow
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked in 1% formaldehyde, lysed and then sheared chromatin was immunoprecipitated with antibodies targeting histone marks [H3K36me2 (Abcam, ab9049), H3K27me3 (Abcam, ab6002), H3K27ac (Abcam, ab4729) and H3K9ac (Millipore, 06-942)]. A small aliquot was set aside prior to immunoprecipitation as input DNA. Quality control was performed using an Agilent Technologies 2100 Bioanalyzer prior to library preparation. KAPA HyperPrep Kit was used for library construction