Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KMT2B

Cell type

Cell type Class
Others
Cell type
VDH-15
NA
NA

Attributes by original data submitter

Sample

source_name
VDH-15_post-PA 14D_MLL2_ChIPSeq
cell line
VDH-15
cell type
oral squamous cell carcinoma (OSCC) cell line
shRNA
control pLKO.1
treatment
post-PA 14D
chip antibody
MLL2 in-house antibody

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were obtained, nuclei sonicated and histone-DNA complexes isolated with antibody according to the ChIP-IT High Sensitivity kit® from Active Motif (ref. 53040). Libraries were prepared according to Illumina's instructions accompanying the NEBNext Ultra DNA Library Prep kit (Ref.:#7370).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
65835190
Reads aligned (%)
96.1
Duplicates removed (%)
33.4
Number of peaks
29101 (qval < 1E-05)

hg19

Number of total reads
65835190
Reads aligned (%)
95.9
Duplicates removed (%)
33.6
Number of peaks
29025 (qval < 1E-05)

Base call quality data from DBCLS SRA