GSM1567514: T(HCC)-P17; Homo sapiens; Bisulfite-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Bisulfite-Seq
Antigen
Bisulfite-Seq
Cell type
Cell type Class
Liver
Cell type
Hepatocellular carcinoma
NA
NA
Attributes by original data submitter
Sample
source_name
hepatocellular carcinoma (HCC) tissue
patient id
P17
tissue
hepatocellular carcinoma (HCC) tissue
Sequenced DNA Library
library_strategy
Bisulfite-Seq
library_source
GENOMIC
library_selection
RANDOM
library_construction_protocol
Genomic DNA of tissue samples, WBC or cancer cell lines including the human liver hepatocellular carcinoma cell line (HePG2) and the human cervical cancer cell line (HeLa) were extracted using the DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer’s protocol.The fully methylated human genomic DNA is purchased (Chemicon/Millipore, CpGenome Universal Methylated DNA, S7821).The plasma cell-free DNAs were extracted using the QIAamp DNA Blood Midi Kit (Qiagen) according to the manufacturer’s protocol. 1) bisulfite treatment of the DNA, 2) a single-tube three-step amplification of methylated CpG islands, 3) gel-purification of the PCR product that serves as the final sequencing library, and 4) high-throughput sequencing using a next-generation sequencer.