Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RELA

Cell type

Cell type Class
Adipocyte
Cell type
SGBS
NA
NA

Attributes by original data submitter

Sample

source_name
Adipocyte
cell type
SGBS adipocyte D10
chip antibody
RELA (C-20, sc-372, Santa Cruz)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed according to standard protocol as described in (Siersbæk et al. 2012, MCB, 32: 3452-3463) Libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014, Methods in Enzymology 2014, Vol. 537, pp. 261-279).

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg19

Number of total reads
20117880
Reads aligned (%)
66.9
Duplicates removed (%)
28.3
Number of peaks
13724 (qval < 1E-05)

hg38

Number of total reads
20117880
Reads aligned (%)
68.1
Duplicates removed (%)
27.5
Number of peaks
13555 (qval < 1E-05)

Base call quality data from DBCLS SRA