Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
T cells
cell type
CD4+
genotype
IRF5+/+
treatment
72 hour activated

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Nuclei were incubated in transposition mix (TD reaction buffer, Nextera Tn5 Transposase [Illumina] at 37oC for 30min and then purified with MinElute PCR purification (Qiagen) Libraries were prepared with the Nextera XT DNA Library Preparation Kit (cat# FC-131-1001) according to manufacturer instructions and then purified with MinElute PCR purification (Qiagen).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
51121845
Reads aligned (%)
63.9
Duplicates removed (%)
29.2
Number of peaks
22064 (qval < 1E-05)

mm9

Number of total reads
51121845
Reads aligned (%)
63.8
Duplicates removed (%)
29.3
Number of peaks
21995 (qval < 1E-05)

Base call quality data from DBCLS SRA