pTO-Klf13Bio plasmid to express a biotinylated form of KLF13
ip protocol
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed, crosslinked with 1 % paraformaldehyde and chromatin extracted and sonicated using an M220 Focused-Ultrasonicator (Covaris) for 20 min with a 5 % duty factor. The chromatin shearing (200-600 bp) was confirmed by agarose gel electrophoresis. DNA libraries were prepared by the University of Michigan DNA Sequencing Core.