Antigen

Antigen Class

TFs and others

Antigen

FOXA1

Cell type

Cell type Class

Breast

Cell type

CAMA-1

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

CAMA1 cells

cell line

CAMA1

cell type

ER+/FGFR1-amplified breast cancer cells

passage

less than 10

antibody

Anti-HNF-3α (Q6) (sc - 101058)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody conjugated with Gammabind G sepharose beads. Libraries from ChIPed DNA were prepared using KAPA Hyper Library Preparation Kit. Samples were end repaired, 3' ends adenylated and barcoded with multiplex adapters, followed by size selection with Ampure XP beads and PCR amplification. Samples were next quantified by Qubit and validated on the Agilent Tapestation 4200, normalized and pooled, and then run on the Illumina NextSeq 500 using 75 cycle SBS v2.5 reagents

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

53348815

Reads aligned (%)

94.9

Duplicates removed (%)

11.9

Number of peaks

66675 (qval < 1E-05)

hg19

Number of total reads

53348815

Reads aligned (%)

94.3

Duplicates removed (%)

12.1

Number of peaks

67622 (qval < 1E-05)