Cells were resuspended in lysis buffer, centrifugated and resuspended for 60 min at 37℃ in Transposase reaction mix. The fragmented DNA was purified and added adaptor sequences by PCR. After DNA purification, DNA was sequenced on HiSeq X ten. Construction of libraries were performed using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme).