Antigen

Antigen Class

TFs and others

Antigen

TBL1Y

Cell type

Cell type Class

Blood

Cell type

DLBCL

NA

NA

Sample

source_name

human DLBCL cell line

lymphoma cell line

Pfeiffer

cell type

DLBCL cell line

chip antibody

anti-TBL1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell pellets were snap frozen and shipped on dry ice to Active Motif where subsequent steps were performed according to previously published pipeline (de Almeida Nagata DE et al. Cell Rep 2019;27(1):269-81) Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. Chip-Seq: After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500 (75 nt reads, single end).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

36365300

Reads aligned (%)

85.3

Duplicates removed (%)

69.1

Number of peaks

1680 (qval < 1E-05)

hg19

Number of total reads

36365300

Reads aligned (%)

84.9

Duplicates removed (%)

70.3

Number of peaks

1576 (qval < 1E-05)