Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Breast
Cell type
NMuMG
Tissue
Mammary Gland

Attributes by original data submitter

Sample

source_name
NMuMG cells
cell line
NMuMG
genotype/variation
overexpressing BC030870
chip antibody
anti-H3K27Ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were extracted according to the Magna-ChIP (Millipore) protocol Libraries were constructed using the ChIP Elute Kit and DNA SMART ChIP-Seq Kit (Illumina) sequencing library preparation from low-input DNA templates, 75nt paired-end

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
12726197
Reads aligned (%)
94.0
Duplicates removed (%)
29.0
Number of peaks
17400 (qval < 1E-05)

mm9

Number of total reads
12726197
Reads aligned (%)
93.5
Duplicates removed (%)
29.1
Number of peaks
17428 (qval < 1E-05)

Base call quality data from DBCLS SRA