Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Breast

Cell type

NMuMG

Tissue

Mammary Gland

Sample

source_name

NMuMG cells

cell line

NMuMG

genotype/variation

overexpressing BC030870

chip antibody

anti-H3K27Ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were extracted according to the Magna-ChIP (Millipore) protocol Libraries were constructed using the ChIP Elute Kit and DNA SMART ChIP-Seq Kit (Illumina) sequencing library preparation from low-input DNA templates, 75nt paired-end

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

32047088

Reads aligned (%)

94.3

Duplicates removed (%)

37.1

Number of peaks

31262 (qval < 1E-05)

mm9

Number of total reads

32047088

Reads aligned (%)

93.9

Duplicates removed (%)

37.1

Number of peaks

31274 (qval < 1E-05)