GSM4455350: LCL1-E2F6 rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
E2F6
Cell type
Cell type Class
Blood
Cell type
Lymphoblastoid cell line
Tissue
blood
Lineage
mesoderm
Description
parental cell type to lymphoblastoid cell lines
Attributes by original data submitter
Sample
source_name
lymphoblastoid cell line
cell type
EBV-transformed LCLs
chip-seq antibody
E2F6 (sc-22823X; Santa Cruz)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
30 million LCL1 cells were crosslinked with 1% formaldehyde and harvested for sonication. Then cell lysates including sheared genomic DNA were immunoprecipitated with the indicated antibody (anti-E2F1, anti-E2F6, anti-Rb, anti-HDAC1, and anti-HDAC2) or normal IgG to collect the DNA fragments that can bind with the interest proteins. The immune complexes were incubated with the salmon sperm DNA/protein A agarose slurry, and the collected DNAs were purified for further processes. The DNA library was prepared with TruSeq ChIP Library Preparation Kit (IP-202-1012; Illumina, San Diego, CA). Then the purified DNAs were sequenced using the Illumina HiSeq platform by the Genome Technology Access Center (GTAC) at the Washington University in St. Louis.