Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pancreas

Cell type

Islets of Langerhans

MeSH Description

Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.

Sample

source_name

H3K4me3-2wk-IUGR-1

cell type

Pancreatic islets

condition

IUGR

time point

2 weeks

chip antibody

H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with different antibodies. Multiplexed libraries were created using the Illumina ChIP-seq DNA sample prep kit following manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

rn6

Number of total reads

62885475

Reads aligned (%)

95.9

Duplicates removed (%)

26.9

Number of peaks

37452 (qval < 1E-05)