Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CPSF3

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293 CDK12as
cell line
HEK293 CDK12as
antibody
A301-091A, Bethyl Laboratories
treatment
NM 15 minutes

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA were purified from chromatin IP products. Total RNA were purified from immunoprecipitated RNA polymerase II. For ChIP-seq, libraries were prepared using Illumina's TruSeq ChIP library Preparation Kit according to Illumina's instructions. For mNET-seq, libraries were prepared using NEBNext® Small RNA Library Prep Set for Illumina, according to NEB's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
33627951
Reads aligned (%)
112.3
Duplicates removed (%)
33.5
Number of peaks
7584 (qval < 1E-05)

hg38

Number of total reads
33627951
Reads aligned (%)
113.8
Duplicates removed (%)
33.2
Number of peaks
7735 (qval < 1E-05)

Base call quality data from DBCLS SRA