GSM4437263: HEK 293T cells, input (ChIP-seq); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK 293T cells
cell line
HEK 293T cells
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The chromatin fraction was prepared according to the fanChIP method described in our previous report [Nucleic Acids Research 42:7 4241-4256 (2014)], and DNA/protein complexes were isolated by anti-HA antibody (3F10, Roche). The fanChIP libraries were constructed using a TruSeq ChIP Sample Prep Kit [iIllumina] following the manufacturer's instructions. The quality and quantity of these libraries were checked using Agilent TapeStation D1000 and KAPA Library Quantification Kits [KAPA BioSystems] / Real-time PCR Systems Step One Plus [Applied Biosystems]. The libraries were sequenced on the Illumina HiSeq 2500 System with 50-bp single-end reads according to the manufacturer's protocol at the core facility of Hiroshima University.