GSM4432224: ChIP CTCFL ID M1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Epitope tags
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
CTCFL ID + Mock treatment
tissue
Mouse embryonic stem cells
parental cell line
E14Tg2a
strain
129/Ola, XY
genotype/variation
CTCF-AID, Tir1, Flag-CTCFL-mRuby 2 transgene at Tigre
kit
Nextera
chip antibody
Anti-Flag M2 Magnetic Beads; Sigma (M8823)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA was extracted using RNeasy Mini Kit (Qiagen 74104) as per the manufacturer's protocol. ChIP-seq libraries were constructed using the Illumina Nextera DNA Sample prep kit. RNA seq libraries were prepared using standard Illumina protocols. Hi-C libraries were prepared as per the Arima kit guidelines.