ChIP-Atlas: SRX7988714

Antigen

Cell type Class: Neural
Cell type: Microglia
MeSH Description: The third type of glial cell, along with astrocytes and oligodendrocytes (which together form the macroglia). Microglia vary in appearance depending on developmental stage, functional state, and anatomical location; subtype terms include ramified, perivascular, ameboid, resting, and activated. Microglia clearly are capable of phagocytosis and play an important role in a wide spectrum of neuropathologies. They have also been suggested to act in several other roles including in secretion (e.g., of cytokines and neural growth factors), in immunological processing (e.g., antigen presentation), and in central nervous system development and remodeling.

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: ATAC-seq libraries were generated as previously described with minor modifications (Buenrostro et al., 2013). In brief, approximately 50,000 sorted cells were washed and lysed. Then, nuclei were tagmented by incubation with Tn5 for 30 min at 37°C. Tagmented genomic DNA was then amplified by PCR for 15 cycles. The libraries were size-selected (100–600 bp) by Ampure XP beads (Beckman Coulter). The concentrations of the final libraries were quantified by Qubit (Thermo Fisher), and fragment lengths were quantified by Fragment Analyzer (Advanced Analytical). The pooled libraries were paired-end sequenced on a NextSeq 500.