Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Digestive tract

Cell type

Gastric cancer cell line

NA

NA

Sample

source_name

Gastric cancer cell line

cell type

EBV infected cell

chip antibody

H3K27ac_ChIP (Active Motif, 91193, lot 01518001)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, sonicated nuclear fraction was incubated with each antibody and histone-DNA complexes were isolated. ChIP-seq libraries were prepared using KAPA Hyper Prep Kit (KAPA Biosystems) following the manufacturer's protocol.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

21089340

Reads aligned (%)

97.7

Duplicates removed (%)

6.5

Number of peaks

27343 (qval < 1E-05)

hg19

Number of total reads

21089340

Reads aligned (%)

97.2

Duplicates removed (%)

7.2

Number of peaks

28200 (qval < 1E-05)