Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Digestive tract

Cell type

Gastric cancer cell line

NA

NA

Sample

source_name

Gastric cancer cell line

cell type

Gastric cancer cell line

chip antibody

H3K4me3_ChIP (Active Motif, 39159, lot 25817005)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, sonicated nuclear fraction was incubated with each antibody and histone-DNA complexes were isolated. ChIP-seq libraries were prepared using KAPA Hyper Prep Kit (KAPA Biosystems) following the manufacturer's protocol.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

19982406

Reads aligned (%)

97.1

Duplicates removed (%)

9.0

Number of peaks

17865 (qval < 1E-05)

hg19

Number of total reads

19982406

Reads aligned (%)

96.6

Duplicates removed (%)

9.8

Number of peaks

18526 (qval < 1E-05)