Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Atm

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
Mouse Embryonic Fibroblast (MEF)
cells
Mouse Embryonic Fibroblast (MEF)
embryo stage
E15.5
passages
2_to_4
strain
C57BL/6
genotype/variation
Hmga2 wt
chip antibody
ATM (Santa Cruz, cat: sc-23922, lot: E3111)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated chromatin and Protein-DNA complexes were isolated with the antibody. Libraries were prepared according to Illumina's instructions accompanying the Ovation Ultra Low Kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
32000000
Reads aligned (%)
95.4
Duplicates removed (%)
15.3
Number of peaks
300 (qval < 1E-05)

mm9

Number of total reads
32000000
Reads aligned (%)
95.2
Duplicates removed (%)
15.3
Number of peaks
253 (qval < 1E-05)

Base call quality data from DBCLS SRA