Antigen

Antigen Class

TFs and others

Antigen

RAD21

Cell type

Cell type Class

Uterus

Cell type

HEC-1-B

Primary Tissue

Uterus

Tissue Diagnosis

Adenocarcinoma Endometrioid

Sample

source_name

HEC-1-B

cell type

human endometrial adenocarcinoma

genotype

mE1

antibody

Rad21 (Abcam, ab992)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to NEB's instructions accompanying the DNA Sample Kit (#E7645).

Sequencing Platform

instrument_model

HiSeq X Ten

hg38

Number of total reads

15584361

Reads aligned (%)

72.1

Duplicates removed (%)

9.8

Number of peaks

25355 (qval < 1E-05)

hg19

Number of total reads

15584361

Reads aligned (%)

71.6

Duplicates removed (%)

10.6

Number of peaks

25254 (qval < 1E-05)