Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Blood

Cell type

Monocytes

MeSH Description

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Sample

source_name

sorted monocytes from peripheral blood

response to decitabine

Non-responder

age

55

gender

male

type of cmml

CMML-1

karyotype

47, XY, +8 [20]

mutation status

Mutant

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

Genomic DNA were isolated using the AllPrep DNA/RNA kit from Qiagen (Valencia, CA) according to manufacturer’s instructions ERRBS libraries were constructed as described in 44. Akalin A et al. Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemia. PLoS Genet. 2012;8(6):e1002781

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

118475271

Reads aligned (%)

73.7

Coverage rate (×)

5.3

Number of hyper MRs

132265 (qval < 1E-05)

hg19

Number of total reads

118475271

Reads aligned (%)

73.7

Coverage rate (×)

6.0

Number of hyper MRs

135879 (qval < 1E-05)