Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Blood

Cell type

Monocytes

MeSH Description

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Sample

source_name

sorted monocytes from peripheral blood

response to decitabine

Responder

age

66

gender

male

type of cmml

CMML-2

karyotype

46, XY, del20 (q11q13) [19]/46, XY [3]

mutation status

Mutant

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

Genomic DNA were isolated using the AllPrep DNA/RNA kit from Qiagen (Valencia, CA) according to manufacturer’s instructions ERRBS libraries were constructed as described in 44. Akalin A et al. Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemia. PLoS Genet. 2012;8(6):e1002781

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

127624063

Reads aligned (%)

68.0

Coverage rate (×)

4.4

Number of hyper MRs

160218 (qval < 1E-05)

hg19

Number of total reads

127624063

Reads aligned (%)

68.1

Coverage rate (×)

5.0

Number of hyper MRs

164290 (qval < 1E-05)