Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Blood

Cell type

Monocytes

MeSH Description

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Sample

source_name

sorted monocytes from peripheral blood

response to decitabine

Non-responder

age

80

gender

male

type of cmml

CMML-2

karyotype

46, XY, del(9) (p21)[3]/46, XY[18]

mutation status

Mutant

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

Genomic DNA were isolated using the AllPrep DNA/RNA kit from Qiagen (Valencia, CA) according to manufacturer’s instructions ERRBS libraries were constructed as described in 44. Akalin A et al. Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemia. PLoS Genet. 2012;8(6):e1002781

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

111119120

Reads aligned (%)

45.5

Coverage rate (×)

2.6

Number of hyper MRs

141478 (qval < 1E-05)

hg19

Number of total reads

111119120

Reads aligned (%)

45.6

Coverage rate (×)

3.0

Number of hyper MRs

145160 (qval < 1E-05)