Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with either anti-NACA or naïve IgG antibodies. ChIP-Seq libraries from immunoprecipitated DNA were constructed using the TruSeq gDNA library kit from Illumina (San Diego, CA) following the manufacturer's instructions. Single reads of 50 base pairs were obtained using a HiSeq 2000 sequencing system (Illumina).