Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Placenta

Cell type

Cytotrophoblast

NA

NA

Sample

source_name

cytotrophoblast

subject id

SUB38

cancer status

non-cancer

organ

placenta, maternal

library

TruSeq DNA Methylation Kit

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

DNA extracted using phenol-chloroform protocol after RNAse treatment. DNA was bisulfite converted with the EZ DNA methylation kit (Zymo). For Nextera transposase-based library approach, genomic DNA was first tagmented using Nextera XT transposome and end repair was performed using 5mC. After bisulfite conversion, Illumina adapters and custom bisulfite converted adapters are attached by limited cycle PCR. Illumina TruSeq DNA Methylation Kit and ACCEL-NGS Methyl-Seq DNA Library kit were used according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

1050941940

Reads aligned (%)

89.5

Coverage rate (×)

29.1

Number of hyper MRs

311532 (qval < 1E-05)

hg19

Number of total reads

1050941940

Reads aligned (%)

89.6

Coverage rate (×)

32.4

Number of hyper MRs

297446 (qval < 1E-05)