ChIP-Atlas: SRX7894779

Antigen

Cell type Class: Blood
Cell type: Monocytes
MeSH Description: Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: RNA-seq: Cell lysates were frozen in RLT plus buffer at -80oC prior to processing. ATAC-seq: cells were processed immediately For RNA-Seq: total RNA was extracted using the RNEasy plus micro kit (Qiagen). Cells were reuspended in RLT plus buffer and stored briefly at – 80 °C prior to extraction. All reagents, plastic ware, and supplies used were sterile, nuclease free, and of molecular biology grade. RNA purity (A260/A280>1.80) and concentration were determined using nanodrop spectrophotometer (Thermo Fisher scientific, Delaware USA). RNA integrity was studied using the 2100 Bioanalyzer System (Agilent) and RNA 6000 Nano Kit. A RNA Integrity Number (RIN) of 6.3 was the lowest cut-off for RNA Sequencing. For ATAC-seq, cells were processed immediately as described (Corces et al (2017), Nat. Methods, 14, 959) RNA-seq libraries were constructed using the Stranded total RNA-seq v2-pico kit (Takara) and ATAC-seq libraries were constructed as described (Corces et al (2017), Nat. Methods, 14, 959)