ChIP-Atlas: SRX7894772

Antigen

Cell type Class: Blood
Cell type: Macrophages
MeSH Description: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: RNA-seq: Cell lysates were frozen in RLT plus buffer at -80oC prior to processing. ATAC-seq: cells were processed immediately For RNA-Seq: total RNA was extracted using the RNEasy plus micro kit (Qiagen). Cells were reuspended in RLT plus buffer and stored briefly at – 80 °C prior to extraction. All reagents, plastic ware, and supplies used were sterile, nuclease free, and of molecular biology grade. RNA purity (A260/A280>1.80) and concentration were determined using nanodrop spectrophotometer (Thermo Fisher scientific, Delaware USA). RNA integrity was studied using the 2100 Bioanalyzer System (Agilent) and RNA 6000 Nano Kit. A RNA Integrity Number (RIN) of 6.3 was the lowest cut-off for RNA Sequencing. For ATAC-seq, cells were processed immediately as described (Corces et al (2017), Nat. Methods, 14, 959) RNA-seq libraries were constructed using the Stranded total RNA-seq v2-pico kit (Takara) and ATAC-seq libraries were constructed as described (Corces et al (2017), Nat. Methods, 14, 959)