Antigen

Antigen Class

Histone

Antigen

H3

Cell type

Cell type Class

Gonad

Cell type

Granulosa Cells

MeSH Description

Supporting cells for the developing female gamete in the OVARY. They are derived from the coelomic epithelial cells of the gonadal ridge. Granulosa cells form a single layer around the OOCYTE in the primordial ovarian follicle and advance to form a multilayered cumulus oophorus surrounding the OVUM in the Graafian follicle. The major functions of granulosa cells include the production of steroids and LH receptors (RECEPTORS, LH).

Sample

source_name

FACS-sorted granulosa cells

cell type

FACS-sorted granulosa cells

strain

C57BL/6 x CD1

embryonic stage

E13.5

Sex

female

transgenic line

TESM-CFP

chip antibody

H3 Active Motif 39763

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Embryonic gonads were micro-dissected at according stage, removed from the mesonephros and incubated in 0.05% trypsin for 5 minutes at 37C. Gonads were then mechanically dissasociated in PBS/3% BSA and passed through a filter cap to obtain a single cell suspension. Fluorescently-labeled cells were collected by Fluorescence Activated Cell Sorting (FACS). For ChIP-seq, ~100K cells were collected per replicate. ChIP-seq libraries were prepared with no modifications according to Van Galen et al., 2016.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

56188396

Reads aligned (%)

69.0

Duplicates removed (%)

10.1

Number of peaks

341 (qval < 1E-05)

mm9

Number of total reads

56188396

Reads aligned (%)

68.9

Duplicates removed (%)

10.1

Number of peaks

476 (qval < 1E-05)