Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Neural

Cell type

Hippocampus

MeSH Description

A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.

Sample

source_name

hippocampus

strain

C57BL6

genotype

KMT2D+/+

treatment

TAK-418

age

30-day old

chip antibody

H3K4me1(Abcam, ab8895, lot GR3186715)

tissue

hippocampus

index

TTAGGC

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sonicated nuclear lysate were pre-cleared and histone-DNA complex were isolated with anti-H3K4me3 antibody (Abcam, ab8580) and anti-H3k4me1(Abcam, ab8895). After reverse cross-linking, DNA fragment were purified by DNA Clean and Concentrator (Zymo Research) NEBNext UltraII DNA Library Prep kit for Illumina

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

14046457

Reads aligned (%)

69.8

Duplicates removed (%)

11.8

Number of peaks

96 (qval < 1E-05)

mm9

Number of total reads

14046457

Reads aligned (%)

69.7

Duplicates removed (%)

11.7

Number of peaks

67 (qval < 1E-05)