ChIP-Atlas: SRX7884420

Antigen

source_name: WT 9-12_control_Nrf2 activator sulforaphane (SFN)
cell line background: WT 9-12
cell type: immortalized epithelial cell line obtained from patients with ADPKD
genotype/variation: control
treatment: Nrf2 activator sulforaphane (SFN)
chip antibody: Bethyl, A300-793A

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Cells were cross-linked with 1% formaldehyde in 1 mL PBS for 10 min at RT. The crosslink was quenched by adding 67 μL of 2.5 M glycine(150 mM final). Cells were washed with PBS and harvested using ChIP lysis buffer. Cells were then sonicated to obtain fragments (200-500 bp) with Sonicator. Immunoprecipitation was performed with indicated antibodies. After elution and reversal cross-linking, DNA was purified for library preparation. Library construction were performed using VAHTS Universal DNA Library Prep Kit (Vazyme, ND607-02) and VATHS DNA Adapters set2 for Illumina (Vazyme Biotech, Cat.N802-01)