GSM4404139: NUP98-KDM5A AML Input; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
Acute myeloid leukemia
NA
NA
Attributes by original data submitter
Sample
source_name
murine acute myeloid leukemia (AML) cells
cell type
AML cells transformed with FLAG-tagged NUP98-KDM5A
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fixed cell pellets were sonicated in Bioruptor for 45 cycles at high setting to enrich the sonicated DNA to be ~300-500bp. Then these sonicated samples were incubated with indicated antibodies with either protein G or A beads overnight. Then, the beads were washed 4 times with distinct buffers (see our method) and eluted with elution buffer at 65 degree for 30 min. This eluted DNA were reverse crosslinked and treated with RNase and protease K, then the final Chipped DNA are purified with PCR cleanup kit (Qiagen). The ChIP DNA library was prepared following the instructions of Illumina's ChIP-seq prep product manual. ChIP-seq libraries were sequenced on Illumina Genome Analyzer for sequencing.