Antigen

Antigen Class

Histone

Antigen

H2Bub

Cell type

Cell type Class

Neural

Cell type

Sciatic Nerve

MeSH Description

A nerve which originates in the lumbar and sacral spinal cord (L4 to S3) and supplies motor and sensory innervation to the lower extremity. The sciatic nerve, which is the main continuation of the sacral plexus, is the largest nerve in the body. It has two major branches, the TIBIAL NERVE and the PERONEAL NERVE.

Sample

source_name

sciatic nerve P14 (Rnf40 ctrl)

strain

mixed 129SvJ/C57Bl/6J background

age

postnatal day 14

tissue

sciatic nerve

genotype

Rnf40 fl/fl

chip antibody

H2Bub1 (Cell Signaling, 5546)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sciatic nerve lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions. Libraries were sequenced following the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

17659325

Reads aligned (%)

98.9

Duplicates removed (%)

3.2

Number of peaks

289 (qval < 1E-05)

mm9

Number of total reads

17659325

Reads aligned (%)

98.8

Duplicates removed (%)

3.2

Number of peaks

310 (qval < 1E-05)