GSM4377839: KO 6DL UNC1999 n 3; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Tert-/- Embryonic stem cells
strain
129J
passage numbers
p46
treatment group
KO_6DL_UNC1999
sample preparation
ChIP-seq
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ESC populations were harvested and crosslinked in formaldehyde. Cell pellets (frozen, or fresh) were subjected to chromatin preparation and immunopreciptiation with an antibody against H3K27me3, followed by extraction of genomic DNA. DNA samples were quantified, and libraries were generated and amplified by PCR, followed by library size determination using a Agilent BioAnalyzer. Libraries were then pooled, quantified, and sequenced on an Illumina MiSeq to determine the amount required for deep sequencing. Pooled libraries were then diluted accordingly and loaded on an Illumina NovSeq6000 for sequencing.